100 Reaction Kit. For robust two-step RT-PCR and RT-qPCR of RNA up to 14 kb. Transcriptor Reverse Transcriptase – the core component of the kit – is a recombinant reverse transcriptase expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity, and RNase H activity that degrades RNA in RNA:DNA hybrids. The latter circumvents the need to perform an additional time-consuming RNase H incubation step after reverse transcription. This shortens the reaction time and reduces costs. Transcriptor Reverse TranscriptaseTranscriptor RT Reaction Buffer, 5x concentratedProtector RNase InhibitordNTP Mix, PCR GradeAnchored Oligo (dT)18 PrimerRandom Hexamer Primer Control RNA – total RNA fraction purified from the immortalized cell line K-562
Catalog No. 50-997-552
